Wednesday, August 26, 2020

American Civil War in the West - 1863-1865

American Civil War in the West - 1863-1865 The Tullahoma Campaign As Grant was leading activities against Vicksburg, the American Civil War in the West proceeded in Tennessee. In June, subsequent to stopping in Murfreesboro for about a half year, Maj. Gen. William Rosecrans started moving against Gen. Braxton Braggs Army of Tennessee at Tullahoma, TN. Leading a splendid crusade of move, Rosecrans had the option to turn Bragg out of a few protective positions, compelling him to surrender Chattanooga and driving him from the state. Skirmish of Chickamauga Strengthened by Lt. Gen. James Longstreets corps from the Army of Northern Virginia and a division from Mississippi, Bragg laid a snare for Rosecrans in the slopes of northwestern Georgia. Propelling south, the Union general experienced Braggs armed force at Chickamauga on September 18, 1863. Battling started vigorously the next day when Union Maj. Gen. George H. Thomas assaulted Confederate soldiers on his front. For the greater part of the day, battling flooded here and there the lines with each side assaulting and counterattacking. On the morning of the twentieth, Bragg endeavored to flank Thomas position at Kelly Field, with little achievement. In light of the bombed assaults, he requested a general ambush on the Union lines. Around 11:00 AM, disarray prompted a hole opening in the Union line as units were moved to help Thomas. As Maj. Gen. Alexander McCook was endeavoring to plug the hole, Longstreets corps assaulted, abusing the gap and steering the traditional of Rosecrans armed force. Withdrawing with his men, Rosecrans withdrew the field leaving Thomas in order. Excessively intensely drew in to withdrawal, Thomas combined his corps around Snodgrass Hill and Horseshoe Ridge. From these positions his soldiers beat off various Confederate attacks before falling back under the front of dimness. This brave resistance earned Thomas the moniker The Rock of Chickamauga. In the battling, Rosecrans endured 16,170 losses, while Braggs armed force brought about 18,454. Attack of Chattanooga Staggered by the annihilation at Chickamauga, Rosecrans withdrew right back to Chattanooga. Bragg followed and involved the high ground around the city successfully putting the Army of the Cumberland under attack. Toward the west, Maj. Gen. Ulysses S. Award was resting with his military close to Vicksburg. On October 17, he was provided order of the Military Division of the Mississippi and control of every single Union armed force in the West. Moving rapidly, Grant supplanted Rosecrans with Thomas and attempted to revive gracefully lines to Chattanooga. This done, he moved 40,000 men under Maj. Gens. William T. Sherman and Joseph Hooker east to strengthen the city. As Grant was emptying troops into the zone, Bragg numbers were diminished when Longstreets corps was requested away for a crusade around Knoxville, TN. Clash of Chattanooga On November 24, 1863, Grant started tasks to drive Braggs armed force away from Chattanooga. Assaulting at sunrise, Hookers men drove Confederate powers from Lookout Mountain south of the city. Battling here finished around 3:00 PM when ammo came up short and a substantial haze wrapped the mountain, winning the face the moniker Conflict Above the Clouds. At the opposite stopping point, Sherman propelled taking Billy Goat Hill at the north finish of the Confederate position. The next day, Grant made arrangements for Hooker and Sherman to flank Braggs line, permitting Thomas to progress up the substance of Missionary Ridge in the inside. As the day advanced, the flank assaults got hindered. Feeling that Bragg was debilitating his middle to strengthen his flanks, Grant requested Thomas men to push ahead to ambush the three lines of Confederate channels on the edge. In the wake of making sure about the primary line, they were nailed somewhere around fire from the staying two. Ascending, Thomas men, without orders, proceeded up the incline, reciting Chickamauga! Chickamauga! what's more, penniless the focal point of Braggs lines. With no decision, Bragg requested the military to withdraw back to Dalton, GA. Because of his annihilation, President Jefferson Davis diminished Bragg and supplanted him with Gen. Joseph E. Johnston. Changes in Command In March 1964, President Abraham Lincoln elevated Grant to lieutenant general and set him in preeminent order of every single Union armed force. Withdrawing Chattanooga, Grant gave order to Maj. Gen. William T. Sherman. A long-term and confided in subordinate of Grants, Sherman promptly made arrangements for driving on Atlanta. His order comprised of three armed forces which were to work in show: the Army of the Tennessee, under Maj. Gen. James B. McPherson, the Army of the Cumberland, under Maj. Gen. George H. Thomas, and the Army of the Ohio, under Maj. Gen. John M. Schofield. The Campaign for Atlanta Moving southeast with 98,000 men, Sherman initially experienced Johnstons 65,000-man armed force close to Rocky Face Gap in northwest Georgia. Moving around Johnstons position, Sherman next met the Confederates at Resaca on May 13, 1864. In the wake of neglecting to break Johnstons protections outside the town, Sherman again walked around his flank and constrained the Confederates to fall back. Through the rest of May, the Sherman consistently moved Johnston back towards Atlanta with fights happening at Adairsville, New Hope Church, Dallas, and Marietta. On June 27, with the streets too sloppy to even think about stealing a walk on the Confederates, Sherman endeavored to assault their situations close Kennesaw Mountain. Rehashed ambushes neglected to take the Confederate entrenchments and Shermans men fell back. By July 1, the streets had improved permitting Sherman to again move around Johnstons flank, dislodging him from his entrenchments. The Battles for Atlanta On July 17, 1864, tired of Johnstons consistent retreats, President Jefferson Davis provided order of the Army of Tennessee to the aggressive Lt. Gen. John Bell Hood. The new authorities first move was toâ attack Thomas armed force close Peachtree Creek, upper east of Atlanta. A few decided ambushes struck the Union lines, yet were eventually totally shocked. Hood next pulled back his powers to the inward protections of the city trusting Sherman would follow and free himself up to assault. On July 22, Hoodâ assaulted McPhersons Army of the Tennesseeâ on the Union left. After the assault made beginning progress, moving up the Union line, it was halted by massed ordnance and counterattacks. McPherson was murdered in the battling and supplanted with Maj. Gen. Oliver O. Howard. Unfit to enter the Atlanta resistances from the north and east, Sherman moved toward the west of the city however was hindered by the Confederates at Ezra Churchâ on July 28. Sherman next chose to constrain Hood from Atlanta by cutting the railways and gracefully lines into the city. Pulling nearly of his powers from around the city, Sherman walked on Jonesborough toward the south. On August 31, Confederate troopsâ attacked the Union positionâ but were effectively determined away. The following day Union soldiers counterattacked and got through the Confederate lines. As his men fell back, Hood understood that the reason was lost and started emptying Atlanta the evening of September 1. His military withdrew west towards Alabama. In the crusade, Shermans armed forces endured 31,687 losses, while the Confederates under Johnston and Hood had 34,979. Clash of Mobile Bay As Sherman was surrounding Atlanta, the US Navy was directing tasks against Mobile, AL. Driven by Rear Admiral David G. Farragut, fourteen wooden warships and four screens ran past Forts Morgan and Gaines at the mouth of Mobile Bay and assaulted the ironclad CSS Tennessee and three gunboats. In doing as such, they went close to a torpedo (mine) field, which asserted the screen USS Tecumseh. Seeing the screen sink, the boats before Farraguts leader stopped, making him broadly shout Damn the torpedoes! No holding back! Proceeding into the straight, his armada caught CSS Tennessee and shut the port to Confederate delivery. The triumph, combined with the fall of Atlanta, enormously helped Lincoln in his re-appointment crusade that November. Franklin Nashville Campaign While Sherman rested his military at Atlanta, Hood arranged another battle intended to cut the Union gracefully lines back to Chattanooga. He moved west into Alabama wanting to bring Sherman into following, before turning north towards Tennessee. To counter Hoods developments, Sherman dispatched Thomas and Schofield back north to secure Nashville. Walking independently, Thomas showed up first. Hood seeing that the Union powers were isolated, moved to crush them before they could focus. Skirmish of Franklin On November 29, Hood about caught Schofields power close to Spring Hill, TN, yet the Union general had the option to remove his men from the snare and arrive at Franklin. After showing up they involved fortresses on the edges of town. Hood showed up the next day and propelled a huge frontal attack on the Union lines. At times alluded to as the Picketts Charge of the West, the assault was rebuffed with overwhelming setbacks and six Confederate officers dead. Clash of Nashville The triumph at Franklin permitted Schofield to arrive at Nashville and rejoin Thomas. Hood, regardless of the injured state of his military, sought after and showed up outside the city on December 2. Safe in the citys resistances, Thomas gradually arranged for the up and coming fight. Under huge tension from Washington to polish off Hood, Thomas at last assaulted on December 15. Following two days of attacks, Hoods armed force disintegrated and broke down, adequately demolished as a battling power. Shermans March to the Sea With Hood involved in Tennessee, Sherman arranged his crusade to take Savannah. Accepting the Confederacy would possibly give up if its ability for making war was devastated, Sherman requested his soldiers to direct a complete burned earth crusade, obliterating everything in their way. Withdrawing Atlanta

Saturday, August 22, 2020

Themes inU.S and Wold History Essay Example | Topics and Well Written Essays - 500 words - 1

Subjects inU.S and Wold History - Essay Example Before the period of industrialization nuclear families had more opportunity for times of unwinding and family contact. The production line proprietors needed for additional benefits, this incited them to utilize more specialists and ladies and kids were fused as it was anything but difficult to control them. The working hours were as long as 18 hours, decreasing the family bond. Days involved of for the most part work and rest (Fischer 1996). The general public was influenced by the other outcome that was urbanization; this is the way wherein individuals relocated to regions where the processing plants were set. The work power was enormous and couldn't do the trick all the individuals and urbanization wrongdoings like prostitution, burglary came to presence influencing the general public unfavorably. Ascent of private enterprise can be identified with the principal mechanical insurgency. This on the grounds that the expanded utilization of surplus cash for interest in dares to make benefit. This made the middleclass to give authority to the monetary upheaval. From the meaning of industrialist by Karl Marx, ‘capitalists’ were the endeavor business visionaries of the day who frequently took a chance with their riches on recently shaped organizations and ventures through speculation. From this the possibility of private enterprise can be followed to the primary modern upheaval. The plants set with recently discovered apparatus that made creation quicker and less expensive. The wealthier people set up industrial facilities in type of organizations or people and pay wages to the representatives. This at that point turns into the financial arrangement of free enterprise. Without free enterprise there could be no presence of such term as socialism. Before industrialization there was no free enterprise and work was done on one’s comfort and step. Industrialization at that point realized private enterprise, hardly any processing plants were set up, and many individuals were working for the proprietors of this production lines. The entrepreneur were the proprietors of these manufacturing plants, the representatives working in the production lines started encountering mistreatment from the proprietors (business people) this provoked

Tuesday, August 11, 2020

How to Know If You Have a Phone Phobia

How to Know If You Have a Phone Phobia Social Anxiety Disorder Coping Print How to Know If You Have a Phone Phobia By Arlin Cuncic Arlin Cuncic, MA, is the author of Therapy in Focus: What to Expect from CBT for Social Anxiety Disorder and 7 Weeks to Reduce Anxiety. Learn about our editorial policy Arlin Cuncic Medically reviewed by Medically reviewed by Steven Gans, MD on August 05, 2016 Steven Gans, MD is board-certified in psychiatry and is an active supervisor, teacher, and mentor at Massachusetts General Hospital. Learn about our Medical Review Board Steven Gans, MD Updated on January 06, 2020 Social Anxiety Disorder Overview Symptoms & Diagnosis Causes Treatment Living With In Children In This Article Table of Contents Expand Overview Symptoms Treatment Options Coping Strategies View All Phone anxiety is a common fear among those with social anxiety disorder (SAD).?? Many people may not like talking on the phone, or may even have a phone fear. But a fear of talking on the phone may actually be considered a phone phobia when your hesitance to make and receive calls causes you to experience symptoms such as severe anxiety, shortness of breath, or a racing heart. Illustration by Jessica Olah, Verywell Overview Those who do not have social anxiety disorder may be afraid to use the phone; they may be more comfortable in direct social interactions, perhaps due to the fact that face-to-face settings allow them to be able to read non-verbal cues, like facial expressions. However, those with social anxiety disorder obviously suffer from the opposite. If you are dealing with this condition, a phone fear may reflect issues you are dealing with regarding interaction with others in general.?? Symptoms If you answer yes to any of these, your phone fear may indeed be a phobia. Before and After Calls Do You: Feel extremely anxious?Delay  making phone calls  due to anxiety?Worry about bothering the other person?Worry about what you will say?Worry about embarrassing yourself?Avoid making calls or having  others call you?Obsess about  what was said after calls? When on the Phone Do You: Shake?Have trouble concentrating?Feel nauseous?Feel your heart race? The fear of making and receiving phone calls can be disruptive to both your personal and professional lives.?? It is important to take phone anxiety seriously. Although answering the phone and making calls may seem like a simple task that everyone should be able to do, if you suffer from  phone phobia, the anxiety can be terrifying and real. Treatment Options Treatment for phone phobia can include cognitive-behavioral therapy (CBT) techniques, such as cognitive restructuring and exposure training. In addition, there are many self-help strategies that you can use to cope with anxiety about using the phone. Cognitive restructuring involves challenging beliefs and replacing negative thoughts with more constructive alternatives.?? For example, if you constantly worry that you will bother the other person when making a phone call, cognitive restructuring might have you consider the evidence that this is actually true. Why would the person answer the phone if he was too busy? Why would he have asked you to call if he didnt want to talk to you? Eventually, you would reach the conclusion that it is unlikely you are bothering the other person or that he doesnt want to speak with you. Exposure training involves the gradual practice of progressively more difficult behaviors???. In the case of phone anxiety, a hierarchy of fears might look something like the one below (listed from easiest to most difficult). Each behavior is practiced until you are comfortable and can move on to the next most difficult one. The 9 Best Online Therapy Programs of 2020 Telephone Fear Hierarchy Call a number that you know will only have a recorded message, like a customer service line.Call a family member or friend that you know well.Call a business and ask a straightforward question, such as when they close.Call someone that you dont know well with a simple question.Call someone that you dont know well about a complicated issue.Make each of the previous types of calls in front of one person.Make each of the previous types of calls in front of a group of people. Your hierarchy might be different depending on whether you find friends or strangers more difficult to talk to, and whether it is more difficult for you to talk on the phone in front of someone else. It may be difficult to create a hierarchy to deal with the fear of answering calls. If you typically avoid answering the phone, one strategy would be to use a caller ID unit to identify who is calling. You could then start by answering calls from people that you are most comfortable with and letting other calls go to voicemail. Eventually, you would progress to answering more difficult calls. Coping Strategies Ideally, you should practice cognitive-behavioral techniques under the supervision of a trained therapist. If meeting with a CBT counselor isnt possible, or if you have already participated in CBT and are looking for additional ways to cope, the following strategies may come in handy. Smile before making and receiving calls. This may sound silly, but it helps you to relax and conveys a sense of pleasantness to the person youre speaking with.Reward yourself after making difficult calls by spending some time doing something that you enjoy.Visualize yourself successfully making or receiving calls. Imagine a positive conversation and feeling good afterward.If you are concerned about interrupting someone when you call, ask whether you are catching the person at a bad time. If the person is in the middle of something, this gives him the chance to offer to call you back.If someone says no or turns down a request, realize that it could be for many reasons that have nothing to do with you. Try not to read too much into the actions of someone else.Do a bit of preparation before making a call, but dont go overboard. Know generally what you are going to say, but try to anticipate that the conversation may not go exactly as you have planned. If there are important points that you need to bring up, make sure to write those down and keep them handy.Realize that you dont always have to answer the phone. If someone is calling you at a bad time, or if you are too anxious to talk, it is acceptable to let calls go to voicemail from time to time.Know that the phone may not always be the best method of communication. If you need to have a record of your conversation or if you want to give the other person time to reflect before responding, email may be the better choice. However, if the issue you need to discuss is complex, emotional, or involves a lot of back-and-forths, calling or meeting face-to-face is best. A Word From Verywell Phone anxiety is difficult but can be overcome. However, if you find that your fear of making and receiving phone calls extends into other areas of your life and that you have fears of social interaction in general, it might be helpful to consult a mental health professional. If you are diagnosed with social anxiety disorder, treatments such as medication or therapy may be offered to you. Situations That Can Trigger Anxiety

Saturday, May 23, 2020

Comparing and Contrasting Clinton and Connerlys Speeches...

Comparing and Contrasting Clinton and Connerly s Speeches on Affirmative Action Bill Clinton was the 42nd President of the United States. Elected in 1992 and again in 1996, Clinton served as President until January of 2001, when George W. Bush became the 43rd President. Ward Connerly is the founder and chairman of the American Civil Rights Institute. He has gained national attention as an outspoken advocate of equal opportunity for all Americans, regardless of race, sex, or ethnic background. In this paper, I will discuss the position of these two politicians from affirmative action, I will highlight how each of them thinks about affirmative action, whether he sees it beneficial or harmful to the American society, and how he argues to†¦show more content†¦He blamed this divisiveness on those who believe that skin color and gender should entitle certain groups of people to some benefits while denying them from other for no reason but racial preferences: Do we not believe it was divisive when those from an earlier period said slavery was immoral? Was it not divisive when our nation s people fought among themselves over this very issue? Was it not divisive when we sent troops into Montgomery and Selma, Alabama to protect the rights of people like Rosa Parks and James Meredith to ensure their right to sit wherever they wanted on a bus and to attend a college that wasn t segregated? Yes, those were divisive times. But the seeds of division are not planted by those of us who seek to eliminate racial and ethnic preferences; they are planted by those who believe that our skin color and gender and how we spell our last names should entitle us to the harvest of diversity ― college admission, government employment, and contracts. (Connerly 5) Clinton began to give many examples of discrimination practices that used to happen as early as 1960s. He explained how blacks was denied attending segregated schools and universities, how the streets in black neighborhood weren t paved, and how the restrooms were marked WHITE and COLORED even in courthouse squares. Most of the powerful positions and even decent jobs were limited to white men: Thirty years ago in this city, you didn

Tuesday, May 12, 2020

The Criminalization Of Prostitution Does More Harm Than...

Thesis: The criminalization of prostitution does more harm than good when often women are left unprotected, both socially and legally, and therefore, the United States should make efforts to decriminalize and regulate prostitution instead. I thought overall my essay turned out pretty good; prostitution was an interesting topic to research and write about. I think my essay could have been a bit better because I was a bit pressed for time these past few weeks with finals, and I didn’t get the chance to have another person read the final product. I think that if I were to revise it again, I would have another person take a look at the final essay and give me some feedback about the strength of my overall argument. I would like to know if it†¦show more content†¦The sex industry is highly divided between those who call themselves â€Å"sex workers†, and former prostitutes who call themselves â€Å"survivors† (Glazer 340). Women should have the right to choos e what they do for a living, including prostitution. The criminalization of prostitution does more harm than good when often women are left unprotected, both socially and legally, and therefore, the United States should make efforts to decriminalize and regulate prostitution instead. Opponents of legalization, contend that that prostitution is â€Å"a degrading and abusive practice that fosters crime, exposes prostitute to violence, and increases the spread of sexually transmitted diseases† (â€Å"Prostitution†). Some maintain that prostitution is not a victimless crime, and it damages women emotionally and physically; for example, street prostitutes experience high levels of post-traumatic stress disorder that are comparable to those among rape survivors, battered women, and survivors of torture, and have high rates of anxiety and depression. They also claim that there is no evidence to support the claim that legalizing prostitution would reduce the prevalence of assault; some analysts even claim that prostitution may foster attitudes that contribute to violence against women (â€Å"Prostitution†). The Coalition against Trafficking in Women claims that â€Å"prostitution affects all women, justifies the sale of any woman, and reduce all wom en to sex†,Show MoreRelatedShould Prostitution Be More Beneficial?1659 Words   |  7 PagesAllie Godat Jessica Perkins Writing 1 November 13, 2015 Prostitution In this essay, I will be giving my arguments on whether or not prostitution would be more beneficial to society and safer it wasn’t illegal. I will be giving you background information on the history of prostitution at the beginning of civilization, and how it came to be illegal in the United States. Next I will state the reasons why prostitution is currently criminalized, and why people think it should remain that way. I willRead MoreThe Effects Of Sex On The United States3198 Words   |  13 Pagesof Criminalizing Sex Work In The United States Sex work and prostitution has long been a taboo topic in many societies, and the United States is not exempt. It is viewed by many people outside of sex work as exploitive, degrading, morally wrong or questionable, and is criminalized in the United States. However, many people who hold these views, including government officials, either are misinformed about the effects of criminalization or simply do not know the extent of negative consequences. AreasRead MoreThe Legal Status Of Prostitution2252 Words   |  10 Pagesoutdated, but rather heavily debated upon over time is prostitution, a rather taboo topic in the 21st century. Defined as â€Å"the business or practice of engaging in sexual relations in exchange for payment† (Webster). As odd as that ‘profession’ may sound to many people, prostitution has been a branch of the sex industry for countless years and, for long, has been deemed â€Å"the world’s oldest profession† (Kipling). Despite its history, prostitution has been criminalized in various parts of the world andRead MoreSex For Cash : Her Call2225 Words   |  9 Pagesregulations women like her were safe and protected to live a safely and to have a dignified life with integrity. She felt proud for what she had accomplished all her own Unfortunately, this scenario is just a fictional tale about a day in the life of a woman who’s right to make decisions regarding her autonomy, continues to be denied, under unchanged and antiquated laws enacted over a century ago on prostitution. These are the same law that was initially created to ‘keep the virtue of proper women’ intactRead MoreSex Trafficking : A Unique Sector Of Slavery1201 Words   |  5 PagesSex trafficking is a unique sector of slavery that primarily affects women and children. This is sexual exploitation for profit where victims face inhumane and volatile conditions. The practice comes in varying forms including brothels, strip clubs, and online services. This horrible occurrence happens secretly in practically any corner across the globe. Although it causes immense physical harm to its victims, the physiological and emotional damage remains severe and prominent. Victims from AsiaRead MoreProstitution and Human Trafficking Essay3070 Word s   |  13 PagesProstitution has been referred to as the oldest profession. Prostitutes can be found in every city and country across the globe. There are a large number of individuals who are currently in the United States and around the world who can be classified as a prostitute. They are expanding from the traditional street level prostitutes to highly paid, high class, prostitutes or escorts. Yet, what exactly would drive those men and women to pursue this class of work, once in this type of work what keepsRead MoreProstitution And Its Effects On Society2159 Words   |  9 PagesIn the United States of America, prostitution is illegal in forty-nine of the fifty states. In most countries worldwide, prostitution is illegal. Despite this, prostitution is still active in every single state, and every single part of the world. Sex work plays a key role of the sexism in American, and worldwide cultures. The criminalization of prostitution has created an underworld of illegal acti vity, that has traumatic consequences on an estimated one million prostitutes. This criminalizing alsoRead MoreThe World s Oldest Profession3990 Words   |  16 Pagescheeseburgers on commercials, and most definitely sells pornography, and movies. Prostitution is a taboo described as the world s oldest profession† worldwide, dating as far back as the 18th century BCE. Although forbidden by many religions, prostitution was practiced commonly by Greeks, Romans, China and other ancient cultures and civilizations. Ironically, it was from Mesopotamian religious practices that sex trade emerged from. Women would offer their ‘sacred’ body parts to men who would offer money toRead MoreEnd The War On Drugs Essay1289 Words   |  6 Pageswar on drugs dates back to 1971, when President Richard Nixon first popularized it. His presidency and administration were primarily responsible for getting the ball rolling. Following his presidency, every administration has more or less doubled down on his stance on the war on drugs. It has become a hot political issue that politicians now use to swing votes by the use of â€Å"fear† and promises of â€Å"safety†. However, the reality is that we are not anymore safe than we were in the 70’s. Drug use isRead MorePoverty and Squatter Syndicates Squatting4474 Words   |  18 Pagesareas in the Philippines. II. Squatting, Squatter Settlements and Squatters Squatting is defined as â€Å"occupying an abandoned or unoccupied space or building, usually residential, that the squatter does not own, rent or otherwise have permission to use.† It has its roots from the end of World War II, when people built makeshift houses called barong-barong inside abandoned private plots of land. Squatting is a major issue in the Philippines, especially in industrialized areas. Statistics in the past

Wednesday, May 6, 2020

Microarray Student Free Essays

In the cell cytoplasm, the ribosome reads the sequence of the Mrs. in groups of three bases to assemble the protein. D. We will write a custom essay sample on Microarray Student or any similar topic only for you Order Now What is the resulting primary sequence Of the protein you made in the interactive? Mennonite, Lucien, Aspartic Acid, Valise, Phenylalanine 2. Go to the â€Å"DNA Microfarad Virtual Lab† found at the university of Tutu’s Learn-Genetics: Genetic Science Learning Center’s website: http://learn. Genetics. Utah. Du/content/labs/microfarad/ 3. Click on the microfarad slide to begin. Then choose Chapter 2 â€Å"Measuring Gene Expression† and go on through Chapter 3 â€Å"The Experiment†. Complete the interactive a first time without writing anything down. 4. Answer the following questions as you work through the virtual DNA microfarad a second time. . What can scientists look for to see which genes are turned on in a particular cell? Provide an example. Scientists use DNA microfarad’s that used techniques allowing the amount of Mrs. transcribed by each gene which allows scientists to determine which genes are expressed to a cell. To do so, they use PC to make copies of a gene and then DNA is placed on the microfarad. An example of this is when the DNA microfarad’s can be used to detect single nucleotide polymorphisms (Snaps). B. Why are tissue samples from healthy and cancer cells taken from the same patient? Both samples are elected to be able to compare the cells, look at differences in gene expression in cells that have the exact genetic blue print. C. How is RNA separated from the rest of the tissue? In order to be separated, RNA samples are mixed with organic solvents, and then they run through a vortex and centrifuge. D. Describe the process used to isolate Mrs. from the other types of RNA. RNA is different from other types of RNA because it is the only type that has Poly-A tails, having several adenine amino acids at the end of the molecule. E. Explain how samples are marked. Cancerous cells are red and healthy cells are green. . Why is it necessary to make a CDMA copy? Why is Mrs. not used? RNA is not used because DNA is a more stable compared to RNA Making a CDMA copy is necessary to visualize the CDMA later on. This is possible because when you make the CDMA copy, you incorporate a fluorescent label in the molecule. G. Draw a diagram of the process of how labeled DNA copies are made. Label the important components of the process. Your diagram should be in color. Turned in. H. What does CDMA stand for and what was used as a template to create it? CDMA stands for complimentary DNA fragments. The reverse transcription of Mrs. allows for single stranded CDMA to be made. I. What happens once you apply the DNA from the two samples to the DNA microfarad? On the microfarad there are many identical copies of the same genes on certain spots, each spot represents a different gene. J. What does the red color indicate? The red color indicates genes that were induced by elevated CA. K. What does the green color indicate? The green color indicates that the gene was strongly repressed by cancer cells. L. What does the yellow color indicate? The yellow color indicates that the gene was neither strongly expressed nor strongly repressed in cancer cells. . What conclusions can you make from microfarad data? Microfarad data is a collection Of DNA spots attached to a solid surface that’s used to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. . Does every red spot mean that the genes in that spot are bad? Explain. Not every red spot is cancerous. Red spots on the merged image represent genes that have had their expression turned up or on in the cancerous tissues and are not expressed in normal cells, the they are not always cancerous. O. What are the limitations of DNA microfarad technology? The quality and amount of RNA remains a ma jor challenge in the microfarad experiments. The amount of obtained tissue and the complexity of the tissue sample itself limit the quality and quantity of RNA that can be isolated. Therefore, clinical studies that are published using the microfarad approach are performed in settings where biological samples are abundant and easily obtainable. 5. Complete the Paper Microfarad analysis that comes with the kit. Part II: Smoking and Lung Cancer Grandpa Joe, Judy Smith’s father, has been a smoker for the past thirty years. Last year, Grandpa Joe came down with a cold that turned into pneumonia. It took him more than a month to recover. The family is very concerned he is going to develop lung cancer. They heard about a study being conducted at the local hospital that is exploring lung-cancer associated genes in smokers and non-smokers. The family convinces Grandpa Joe to participate in the study in order to learn more about his risk for developing lung cancer. The study is investigating six genes thought to be involved with lung cancer using DNA microfarad technology. The researchers hope to compare gene expression of the six genes of interest between smokers and non-smokers in order to gain more knowledge of what causes a normal lung cell to become cancerous. You have been assigned to the study. Your first task is to learn more about the six genes of interest. Below are descriptions for the six genes of interest: Gene Name (and Symbol): Protein Function: Prediction: Gene l: Human carcinogenicity antigen (CANDACE) This gene codes for a protein that is located in the extracurricular matrix. This protein is involved with adhesion between cells and is thought to be a proto- nosecone and when over-expressed is an nosecone. Expressed in smoker. We predict that the CANDACE will progress to the form of a tumor or untie to harbor that potential. Gene 2: Surfactant protein B (SIFTS) This gene codes for an extracurricular protein. This protein enhances the rate of spreading and increases the stability of pulmonary’ surfactant, a lipid-rich material that prevents lung collapse by lowering surface tension at the air- liquid interface in the alveoli of the lungs. Expressed in the Smoker. This gene can possibly progress to spread a higher production of cells or possibly a higher rate of cancer, maybe in the lungs. Gene 3: POP tumor suppressor (HTTP) This gene codes for a protein that is located in the mitochondria and in the nucleolus. This protein is involved with cell cycle checkpoints. This gene is a tumor suppressor gene and is thought to be the â€Å"Guardian of the Genome. † Suppressed in the smoker. I predict this gene is unlikely to get cancer as it monitors cell production, etc. To prevent tumors. Gene 4: CRY This gene codes for a protein that is located in the nucleus. The protein that this gene codes for is testis-determining factor (UDF) which initiates male sex determination. This protein has no function in lung cells. Not expressed in either. Non-smoker-?yellow. This gene probably determines whether or not you get cancer based on cell production. Assume it contributes to getting cancer, unless in the lung cells, where there would be no increase or decrease in likeliness. Gene S: Stockroom IPPP (COPY AY) This gene codes for a protein that is located in the endoplasmic reticulum. The protein catalysts reactions involved in drug metabolism and synthesizes cholesterol, steroids, and other lipids. The expression of this protein is induced by some polycyclic aromatic hydrocarbons (PARS), some of which are found in cigarette smoke. Suppressed in smoker. This gene will contribute to causing cancer if deregulated because it controls cell signaling pathways. Gene 6: Clinical 3(SPEC) This gene codes for a protein that is located in the plasma membrane and extracurricular matrix. The gene controls cellular response to damage and may control cellular growth regulation and apotheosis. This gene is considered to be a tumor suppressor gene for lung cancer. Suppressed in smoker. This gene decreases your risk of cancer as it controls cell self-destruction and responds to damage or advances in cell growth 6. Highlight or underline any interesting or important information about the function of each protein. 7. Predict how these genes will be expressed in a DNA microfarad of a smoker versus a non-smoker. Would you expect the genes to be induced in the smoker (more expressed), suppressed in the smoker (less expressed), not expressed in either the smoker or the non- smoker, or expressed the same in both the smoker and non-smoker? In a DNA microfarad, I would expect these DNA genes to have a bit of diversity. I think Gene 1 and 2 will be likely induced as they can contribute to overall cell production, 4 would be unaffected as Mr.. Smith is testing for lung cancer, and 3, 5, and 6 to be suppressed as they work to control cell production and smoking destroys several of their functions. For example, Gene 6 works to control cell apotheosis– if smoking ruins the chances for a cell to destroy itself, production can get out of control. 8. Record your predictions in the Prediction column of the above table. Part Ill: Microfarad Wet Lab Now that you know more about the six genes of interest, your job is to perform a simulated DNA microfarad using tissue samples taken from Grandpa Joe and a non-smoker’s tissue samples. How to cite Microarray Student, Papers

Saturday, May 2, 2020

Question Of Being Essay Example For Students

Question Of Being Essay annonMany events were unexplainable and maybe even seemed to be magical beforescience evolved to what it is today. All questions relating to the originof life can be answered scientifically. The creation of the universe andall events since can be explained. One may question their beliefs basedon scientific theory. Human life can even be broken down to fundamentaltheory. Not only geological or biological, but all events can be answeredscientifically. One^?s belief in a god or a higher being may help in dealing with the rigorsof life and create a sense of security. All base their lives on some sortof belief. This belief however is different for everyone. The actions of areligious person stem from their belief in a god. A person may act morallyand responsibly in hopes that they will receive good judgement upondeparture from this earth. No one can say for certain if there is a heavenor a hell, but obviously a religious person would rather not go to the laterof the two. This is not to say, that one whom does not believe in a goddoes not act morally or responsibly. An atheist^?s actions do not stem fromthe fear of judgement from god but rather from the judgement of society. Religious and non-religious people alike will most likely base their actionson their conception of the highest ethical good. I consider myself to be sort of an existentialist. This encompasses thescope of individual existence and individual freedom. Life, I believe isbased in its entirety on one goal that is different for everyone. Twothings are ultimately correct, the certainty of death and themeaninglessness of one^?s life. Once a person determines his goal, he mustpursue it with a white-hot passion in order for happiness to be obtained. Camus^? theory of the ^?absurd^? is a belief, which I hold veryhighly. This theory explains that one will spend every moment of his lifechasing the same goal, which he has set for himself, and that life is infact monotonous. In this monotony happiness is reached but can also bequestioned upon the realization of life^?s meaningless pursuit of one goal. At moments in everyone^?s life, they will suddenly become aware of life^?sabsurdity. To one, life may seem meaningless, but looking at the ^?bigpicture^? life is insignificant. While suffering the knowledge that one^?slife is meaningless and entirely monotonous, one must find happiness inlife. This quote from The Myth of Sisyphus by Camus may help to explain howhappiness and the absurd must go hand in hand. ^?Happiness and the absurdare two children of the same earth, they are inseparable.^? If happiness cannot be found in one^?s life then it is not worth living. If one feels they are not subject to the judgement of god, I can only hopethat in their pursuit of happiness they feel the judgement of society. Sadly but truly, there are those among us who feel no moral responsibility. I am uncertain, as is everyone, to what the afterlife will hold. There maybe a heaven and a hell, but there is no concrete evidence supporting this. As is there no evidence supporting the belief that there is no afterlife. Quite a few people wish to live forever and in doing this adopt the idea ofthe afterlife. Camus believed that, ^?Men are never really willing to dieexcept for the sake of freedom: therefore they do not believe in dyingcompletely.^? I believe many people live with the hope that they will diephysically, they will spiritually live forever in the kingdom of heaven. .uc42056a9a60a9068541db2a07f591d20 , .uc42056a9a60a9068541db2a07f591d20 .postImageUrl , .uc42056a9a60a9068541db2a07f591d20 .centered-text-area { min-height: 80px; position: relative; } .uc42056a9a60a9068541db2a07f591d20 , .uc42056a9a60a9068541db2a07f591d20:hover , .uc42056a9a60a9068541db2a07f591d20:visited , .uc42056a9a60a9068541db2a07f591d20:active { border:0!important; } .uc42056a9a60a9068541db2a07f591d20 .clearfix:after { content: ""; display: table; clear: both; } .uc42056a9a60a9068541db2a07f591d20 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .uc42056a9a60a9068541db2a07f591d20:active , .uc42056a9a60a9068541db2a07f591d20:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .uc42056a9a60a9068541db2a07f591d20 .centered-text-area { width: 100%; position: relative ; } .uc42056a9a60a9068541db2a07f591d20 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .uc42056a9a60a9068541db2a07f591d20 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .uc42056a9a60a9068541db2a07f591d20 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .uc42056a9a60a9068541db2a07f591d20:hover .ctaButton { background-color: #34495E!important; } .uc42056a9a60a9068541db2a07f591d20 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .uc42056a9a60a9068541db2a07f591d20 .uc42056a9a60a9068541db2a07f591d20-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .uc42056a9a60a9068541db2a07f591d20:after { content: ""; display: block; clear: both; } READ: Pearl Harbor And FDR EssayMany live their lives in anticipation of an afterlife, which after all maynot exist. The goal, which these people have set for themselves, includesspending an eternity in heaven. Some may ask if in fact, these lives werewasted in pursuit of an afterlife, which may only be fictional. I believethat one^?s life is not wasted if he has found happiness. John Paul Sartreonce said, ^?One is still what one is going to cease to be and already whatone is going to become. One lives one^?s death, one dies one^?s life.^? I don^?t think it is very intelligent to live your life in anticipation of yourdeath. I live my life trying to obtain a goal. Day in and day out I chase afterthis same goal. realizing my life is globally meaningless and monotonous, Ifind happiness in the hope that one day my goal will be reached. Personally, I do not believe in the afterlife, but I do not deny that itcould exist. My actions are made with the judgement of society in mind and,therefore, are moral and responsible according to the expectations of modernsociety. I have found happiness in my life and I feel complete. I do notwish to live forever, but rather I accept that one day I must die, bothphysically and spiritually. My life is not based on the prerequisites setforth by any religion, but rather in obtaining my own personal goal. I willbe able to leave this world happily knowing that I struggled for the samegoal endlessly.